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科学研究

科研团队

微生物药物与过程工程课题组 W

简介
一、课题组简介

针对生物过程中存在的底物不溶、产物抑制、降解等问题，首创浊点系统萃取微生物发酵新技术。目前正在研究的课题包括：1）以甾体微生物转化为例，开发界面生物催化技术；2）应用生物相容化学技术开发红曲色素衍生物及其生物活性。相关研究内容获得3项国家自然科学基金资助，研究成果出版《萃取微生物转化》专著一部、发表了SCI论文50余篇,部分专利成果已开始工业化应用。

二课题组成员简介
1. 负责人
王志龙博士/研究员

教育与研究经历

1988-1992年湘潭大学环境与资源学院学士

1995-1998年天津大学化工学院硕士

2001-2004年欧宝app官方网站下载生命科学技术学院博士

2008年德国赫姆霍兹环境研究中心客座研究员（DAAD基金资助）

2009年美国伊利诺伊大学香槟分校访问学者

1998年-至今欧宝app官方网站下载药学院、生命科学技术学院

2013年-至今微生物代谢国家重点实验室
成员简介

在读研究生
博士生：刘鲁杰（2015级）、谢海胜（2017级）
硕士生：黄瑶琳（2016级）、辛桥（2016级）、顾斌（2017级）、郑嘉唯（2017级）
本科生：格茸培楚（2014级）
毕业研究生
郭雅静（2003级）、王丽（2004级）、张文智（2005级）、梁蕊（2006级）、潘涛（2007级）、薛迎迎（2007级）、戴泽文（2008级）、姚明静（2009级）、胡志强（2010级）、康碧玉（2011级）、熊旭（2012级）、钟思嘉（2013级）、王博（2013级）、胡鸣略（2014级）、赵丽红（2014级）、陆丰翎（2015级）
研究方向/领域

1、生物技术药物：生物催化是合成复杂结构药物或前体的重要手段。应用浊点系统技术解除生物催化（微生物转化与发酵）过程中存在的底物生物利用度低、底物/产物抑制、产物进一步降解等瓶颈问题。
2、微生物次级代谢：微生物次级代谢受到多种环境因素的调控。应用浊点系统萃取发酵技术改变营养物质的吸收和次级代谢产物的分泌，进而调控微生物次级代谢的途径及效率。
主要成果/奖励

2008年欧宝app官方网站下载首届晨星优秀青年学者奖励计划
2016年上海市闵行区领军人才
著作/论文/专利

1. Fengling Lu, Lujie Liu, Yaolin Huang, Xuehong Zhang, Zhilong Wang*. Production of Monascus pigments as extracellular crystals by cell suspension culture. Applied microbiology and biotechnology, 2018, 102(2):1-11
2. Lihong Zhao, Fengling Lu, Xuehong Zhang, Zhilong Wang*. Isolation of ionizable red Monascus pigments after extractive fermentation in nonionic surfactant micelle aqueous solution. Process biochemistry, 2017, 61, 156-162
3. Lihong Zhao, Xuehong Zhang, Zhilong Wang*. Extraction of anionic dyes with ionic liquid-nonionic surfactant aqueous two-phase system. Separation science and technology, 2017, 52(5): 804-811
4. Bo Wang, Xuehong Zhang, Zhenqiang Wu, Zhilong Wang*. Biosynthesis of Monascus pigments by resting cell submerged culture in nonionic surfactant micelle aqueous solution. Applied microbiology and biotechnology, 2016, 100(16):7083-7089
5. Bo Wang, Xuehong Zhang, Zhenqiang Wu*, Zhilong Wang*. Investigation of relationship between lipid and Monascus pigment accumulation by extractive fermentation. Journal of biotechnology, 2015, 212:167-173
6. Sijia Zhong, Xuehong Zhang*, Zhilong Wang*. Preparation and characterization of yellow Monascus pigments. Separation and purification technology. 2015, 150:139-144.
7. Xu Xiong, Xuehong Zhang, Zhenqiang Wu, Zhilong Wang*. Accumulation of yellow Monascus pigments by extractive fermentation in nonionic surfactant micelle aqueous solution. Applied microbiology and biotechnology, 2015, 99 (3): 1173-1180
8. Xu Xiong, Xuehong Zhang, Zhenqiang Wu, Zhilong Wang*. Coupled aminophilic reaction and directed metabolic channeling to red Monascus pigments by extractive fermentation in nonionic surfactant micelle aqueous solution. Process biochemistry, 2015, 50(2): 180-187
9. Lingjie Shen, Xuehong Zhang, Mingzhu Liu, Zhilong Wang*. Transferring of red Monascus pigments from nonionic surfactant to hydrophobic ionic liquid by novel microemulsion extraction. Separation and purification technology, 2014, 138: 34-40
10. Lingjie Shen, Xuehong Zhang, Mingzhu Liu, Zhilong Wang*. Microemulsion extraction of Monascus pigments from nonionic surfactant using high polarity of diethyl ether as excess oil phase. Separation science and technology, 2014, 49: 2346-2351
11. Biyu Kang, Xuehong Zhang, Zhenqiang Wu, Zhilong Wang*, Sunghoon Park. Production of citrinin-free Monascus pigments by submerged culture at low pH. Enzyme and microbial technology, 2014, 55: 50-57
12. Biyu Kang, Xuehong Zhang, Zhenqiang Wu, Hanshi Qi, Zhilong Wang*. Solubilization capacity of nonionic surfactant micelles strong influence on export of intracellular pigments in Monascus fermentation. Microbial biotechnology. 2013, 6(5): 540-550
13. Biyu Kang, Xuehong Zhang, Zhenqiang Wu, Hanshi Qi, Zhilong Wang*. Effect of pH and nonionic surfactant on profile of intracellular and extracellular Monascus pigments. Process biochemistry, 2013, 48: 759-767
联系方式

Researcher. Zhilong Wang

Room 206, Pharmacy Building 7, Minhang campus

Tel. 86-21-3420 8146

Email: zlwang@sjtu.edu.cn
更多信息

Zhilong Wang Lab
1. Introduction
To address issues in the process of biotransformation, including low substrate aqueous solubility, and product inhibition and degradation, we have firstly developed a novel technology concerning extractive microbial transformation in cloud point system. Currently, we are focusing on two major directions: 1) by taking steroid microbial conversions as an example, develop technology of interfacial biocatalysis; 2) by utilizing biocompatible chemistry, exploit potentially valuable red Monascus pigments and evaluate their further biological activity. These research projects are supported by 3 funds of National Natural Science Foundation of China. So far Dr. Wang has published a monograph Extractive Microbial Transformation, and more than 50 articles in SCI journals. Besides, several patents on our research findings have begun to be industrialized.

2. Members
2.1 Principal Investigator
Professor Zhilong Wang

Education and Research Experience
1988-1992 B.S., College of Environment and Resources, Xiangtan University
1995-1998 M.S., School of Chemical Engineering and Technology, Tianjin University
2001-2004 Ph.D., School of Life Sciences and Biotechnology, Shanghai Jiao Tong
University
2008 Visiting Fellow, German Helmholtz Center for Environmental Research
2009 Visiting Scholar, University of Illinois at Urbana-Champaign
1998- Associate/Research Fellow, School of Pharmacy & School of Life Sciences and Biotechnology, Shanghai Jiao Tong University
2013- Research Fellow, State Key Laboratory of Microbial Metabolism (SJTU)

Honors and Awards
Moring-star Awards program for Outstanding Young Scholars from SJTU, 2008
Awards for Leading Talents in Minhang district of Shanghai, 2016

2.2 Graduate Students
Ph.D. candidates: Lujie Liu (2015), Haisheng Xie (2017)
M.S. candidates: Yaolin Huang (2016), Qiao Xin (2016), Bin Gu (2017), Jiawei Zheng (2017)
Undergraduates: Kelsang Phuntsok
Formerly Graduated Students:
Yajing Guo (2003), Li Wang (2004), Wenzhi Zhang (2005), Rui Liang (2006), Tao Pan (2007) Yingying Xue (2007), Wenze Dai (2008), Mingjing Yao (2009), Zhiqiang Hu (2010), Biyu Kang (2011), Xu Xiong (2012), Sijia Zhong (2013), Bo Wang (2013), Minglue Hu (2014), Lihong Zhao (2014), Fengling Lu (2015)

3. Main Research Interests
1, Biopharmaceutics: biocatalysis plays an important role in synthesizing medical products/precursors with complex structures. In biocatalytic process (e.g. microbial conversions and fermentations), cloud point system may be a useful means of dealing with the bottlenecks including limited substrate bioavailability, substrate/product inhibition and further degradation of products.
2, Microbial secondary metabolism: The secondary metabolism of microorganisms can be readily modulated by various environmental factors. Using extractive fermentation in cloud point system, microorganisms’ taking up nutrients and excreting secondary metabolites could be altered, and subsequently modulating the pathways and efficiency of microbial secondary metabolism.

4. Recent Publications
See above, “近期主要研究成果”.
5. Life in Lab
See above, the second and third photos
6. Contact Us
Address: Room 7-205,206, School of Pharmacy, Minhang campus of Shanghai Jiao Tong University, No. 800, Dongchuan Rd, Shanghai, China 200240.
Tel: 021-34208146
E-mail: zlwang@sjtu.edu.cn
Self-motivated students and postdocs are welcome to join us!

简介

成员简介

研究方向/领域

主要成果/奖励

著作/论文/专利

联系方式

更多信息